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Multiple Choice

At pH 8.6, proteins are negatively charged and migrate toward which electrode?

At pH 8.6, proteins carry a net negative charge because the pH is above their isoelectric points. In electrophoresis, particles move toward the electrode with the opposite charge. Negatively charged proteins are attracted to and migrate toward the positively charged electrode, the anode. The cathode is the negative electrode, which would attract positively charged species. So the proteins move toward the anode.

At pH 8.6, proteins carry a net negative charge because the pH is above their isoelectric points. In electrophoresis, particles move toward the electrode with the opposite charge. Negatively charged proteins are attracted to and migrate toward the positively charged electrode, the anode. The cathode is the negative electrode, which would attract positively charged species. So the proteins move toward the anode.