What is a common method to mitigate lipemia when performing photometric assays?

• A. Ultracentrifugation to remove lipids
• B. Do nothing; results are unaffected
• C. Increase sample volume to dilute fats
• D. Use colorimetric assay instead

Answer: (A)

Photometric assays measure how much light a sample absorbs, and lipemia creates turbidity from lipid droplets that scatter light and distort the absorbance reading. Removing the lipids before measurement, typically by ultracentrifugation, clears the sample so the light passes more predictably through the fluid, giving a more accurate result. Doing nothing leaves the interference, diluting fats can alter the analyte concentration and reaction conditions, and simply using a colorimetric approach doesn’t automatically eliminate turbidity effects. So, ultracentrifugation to remove lipids is the effective, commonly used mitigation.