Which dye is more specific for albumin measurement in dye-binding assays?

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Dye-binding albumin assays rely on how selectively the dye binds to albumin versus other serum proteins. Bromcresol Green binds to albumin with high affinity and does so with relatively less interaction with non-albumin proteins, making the measured color change a more accurate reflection of albumin concentration. Bromcresol Purple, on the other hand, tends to interact with a broader range of plasma proteins, increasing non-specific binding and potential interference, which makes it less albumin-specific. The other dyes listed are older options known to have even greater cross-reactivity and interference, so they’re not as specific.